Publication date: Nov 01, 2023
Revolutionary all-in-one RPA-CRISPR assays are rapidly becoming the most sought-after tools for point-of-care testing (POCT) due to their high sensitivity and ease of use. Despite the availability of one-pot methods for specific targets, the development of more efficient methods for new targets remains a significant challenge. In this study, we present a rapid and universal approach to establishing an all-in-one RPA-Cas12a method CORDSv2 based on rational balancing amplification and Cas12a cleavage, which achieves ultrasensitive detection of several targets, including SARS-CoV-2, ASFV, HPV16, and HPV18. CORDSv2 demonstrates a limit of detection (LOD) of 0. 6 cp/μL and 100% sensitivity for SARS-CoV-2, comparable to qPCR. Combining with our portable device(hippo-CORDS), it has a visual detection LOD of 6 cp/μL and a sensitivity up to 100% for SARS-CoV-2 and 97% for Ct
| Concepts | Keywords |
|---|---|
| Bioelectron | Automation design |
| Hpv18 | CRISPR/Cas12 |
| Rapidly | One-pot |
| Viruses | Portable device |
| Virus detection |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | IDO | site |
| disease | VO | Viruses |
| disease | VO | efficient |
| disease | VO | device |