Publication date: Sep 15, 2023
This study investigated the phylogenetic relatedness of multi-drug resistant Elizabethkingia anophelis recovered from an industrial effluent treatment plant (WWTPi). The wastewater samples were plated in Brain Heart Infusion agar (4 mg/L ceftazidime, 8 mg/L meropenem and 2 mg/L polimixin). Four isolates recovered from four stages of WWTPi (influent, aeration, decantation and treated effluent) were identified and evaluated of susceptibility profiles in the VITEK 2 system. These strains identified as E. meningoseptica were confirmed to be E. anophelis by whole genomic sequencing (Miseq-Illumina) and showed antimicrobial resistance genes of β-lactams, aminoglycosides, and tetracycline’s classes. The rMLST showed that they belong to the rST 65620 together with clinical strains. The phylogenomic tree revealed the similarity of our strains to those belonging to sublineage 11 and the SNP analysis confirmed that they belong to a single clade. To the best of our knowledge, this is the first study reporting the persistence of multidrug-resistant E. anophelis sublineage 11 along the wastewater treatment.