FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses.

Publication date: Sep 20, 2023

Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently detecting ex vivo antiviral cytokine production by SARS-CoV-2-specific T cells following peptide stimulation. We then detail intracellular cytokine staining by flow cytometry to further validate the FluoroSpot assay results and define the specific T cell subpopulations. For complete details on the use and execution of this protocol, please refer to Tiezzi et al. (2023). .

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Concepts	Keywords
Antiviral	Cell Biology
Complete	Immunology
Coronavirus
Cytometry

Semantics

Type	Source	Name
disease	IDO	assay
disease	VO	frequency
disease	IDO	host
disease	IDO	immune response
disease	VO	Severe acute respiratory syndrome coronavirus 2
disease	MESH	infection
disease	VO	protocol
disease	VO	immunization
disease	MESH	SARS CoV 2 infection
disease	MESH	Infectious Diseases
disease	IDO	blood
disease	MESH	influenza
disease	IDO	production
disease	VO	storage
drug	DRUGBANK	Dimethyl sulfoxide
disease	VO	vaccinated
disease	VO	dose
disease	VO	vaccination
drug	DRUGBANK	Heparin
drug	DRUGBANK	Diatrizoate
drug	DRUGBANK	Flunarizine
drug	DRUGBANK	Nitrogen
disease	VO	time
disease	VO	USA
drug	DRUGBANK	Phosphate ion
drug	DRUGBANK	Amino acids
drug	DRUGBANK	Methylergometrine
drug	DRUGBANK	Amphotericin B
drug	DRUGBANK	Ethanol
drug	DRUGBANK	L-Glutamine
drug	DRUGBANK	Streptomycin
disease	VO	dead
disease	VO	viability
drug	DRUGBANK	Coenzyme M
drug	DRUGBANK	Water
disease	IDO	quality
disease	VO	volume
disease	IDO	cell
drug	DRUGBANK	Medical air
drug	DRUGBANK	Pirenzepine
disease	MESH	death

Original Article

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