FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses.

FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses.

Publication date: Sep 20, 2023

Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently detecting ex vivo antiviral cytokine production by SARS-CoV-2-specific T cells following peptide stimulation. We then detail intracellular cytokine staining by flow cytometry to further validate the FluoroSpot assay results and define the specific T cell subpopulations. For complete details on the use and execution of this protocol, please refer to Tiezzi et al. (2023). .

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Concepts Keywords
Antiviral Cell Biology
Complete Immunology


Type Source Name
disease IDO assay
disease VO frequency
disease IDO host
disease IDO immune response
disease VO Severe acute respiratory syndrome coronavirus 2
disease MESH infection
disease VO protocol
disease VO immunization
disease MESH SARS CoV 2 infection
disease MESH Infectious Diseases
disease IDO blood
disease MESH influenza
disease IDO production
disease VO storage
drug DRUGBANK Dimethyl sulfoxide
disease VO vaccinated
disease VO dose
disease VO vaccination
drug DRUGBANK Heparin
drug DRUGBANK Diatrizoate
drug DRUGBANK Flunarizine
drug DRUGBANK Nitrogen
disease VO time
disease VO USA
drug DRUGBANK Phosphate ion
drug DRUGBANK Amino acids
drug DRUGBANK Methylergometrine
drug DRUGBANK Amphotericin B
drug DRUGBANK Ethanol
drug DRUGBANK L-Glutamine
drug DRUGBANK Streptomycin
disease VO dead
disease VO viability
drug DRUGBANK Coenzyme M
drug DRUGBANK Water
disease IDO quality
disease VO volume
disease IDO cell
drug DRUGBANK Medical air
drug DRUGBANK Pirenzepine
disease MESH death

Original Article

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