Dynamics of Serum-Neutralizing Antibody Responses in Vaccinees through Multiple Doses of the BNT162b2 Vaccine.

Publication date: Nov 15, 2023

SARS-CoV-2 mRNA vaccines are administered as effective prophylactic measures for reducing virus transmission rates and disease severity. To enhance the durability of post-vaccination immunity and combat SARS-CoV-2 variants, boosters have been administered to two-dose vaccinees. However, long-term humoral responses following booster vaccination are not well characterized. A 16-member cohort of healthy SARS-CoV-2 nacEFve participants were enrolled in this study during a three-dose BNT162b2 vaccine series. Serum samples were collected from vaccinees over 420 days and screened for antigen (Ag)-specific antibody titers, IgG subclass distribution, and neutralizing antibody (nAb) responses. Vaccine boosting restored peak Ag-specific titers with sustained α-RBD IgG and IgA antibody responses when measured at six months post-boost. RBD- and spike-specific IgG4 antibody levels were markedly elevated in three-dose but not two-dose immune sera. Although strong neutralization responses were detected in two- and three-dose vaccine sera, these rapidly decayed to pre-immune levels by four and six months, respectively. While boosters enhanced serum IgG Ab reactivity and nAb responses against variant strains, all variants tested showed resistance to two- and three-dose immune sera. Our data reflect the poor durability of vaccine-induced nAb responses which are a strong predictor of protection from symptomatic SARS-CoV-2 infection. The induction of IgG4-switched humoral responses may permit extended viral persistence via the downregulation of Fc-mediated effector functions.

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Concepts Keywords
Bnt162b2 IgG4 subclass response
Downregulation neutralizing antibodies
Healthy SARS-CoV-2
Months
Vaccine

Semantics

Type Source Name
disease VO vaccine
disease VO effective
disease VO vaccination
disease VO dose
disease MESH SARS-CoV-2 infection
pathway REACTOME SARS-CoV-2 Infection
drug DRUGBANK Amber
disease VO USA
disease VO Glycoprotein
disease VO primary vaccination
disease IDO production
disease MESH Infection
disease IDO blood
drug DRUGBANK Spinosad
drug DRUGBANK Coenzyme M
disease VO protocol
drug DRUGBANK Cobalt
drug DRUGBANK Phosphate ion
disease IDO assay
drug DRUGBANK L-Leucine
drug DRUGBANK Methylergometrine
drug DRUGBANK Amino acids
drug DRUGBANK Streptomycin
disease VO ANOVA
disease VO titer
disease MESH panic
disease VO vaccinee
disease VO vaccine dose
drug DRUGBANK L-Valine
drug DRUGBANK Esomeprazole
drug DRUGBANK Diethylstilbestrol
disease VO Viruses
disease MESH allergy
drug DRUGBANK S-Arsonocysteine
disease VO population
disease VO vaccine strain
disease IDO host
disease MESH asymptomatic infection
disease MESH coronavirus infections
disease VO vaccine efficacy
disease VO effectiveness
disease VO vaccine effectiveness
drug DRUGBANK Angiotensin II
disease IDO cell
disease MESH pollen allergy
disease MESH malignancies
disease IDO pathogen
drug DRUGBANK Guanosine
drug DRUGBANK (S)-Des-Me-Ampa
disease VO vaccinated
disease MESH Breakthrough Infections
drug DRUGBANK Nonoxynol-9

Original Article

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