RSV neutralizing antibodies in dried blood.

Publication date: Nov 30, 2023

The key correlate of protection of respiratory syncytial virus (RSV) vaccines and monoclonal antibodies (mAb) is virus neutralization, measured using sera obtained through venipuncture. Dried blood obtained with a finger prick can simplify acquisition, processing, storage, and transport in trials, and thereby reduce costs. In this study we validate an assay to measure RSV neutralization in dried capillary blood. Functional antibodies were compared between matched serum and dried blood samples from a phase I trial with RSM01, an investigational anti-RSV Prefusion F mAb. Hep-2 cells were infected with a serial dilution of sample-virus mixture using RSV-A2-mKate to determine half-maximal inhibitory concentration. Stability of dried blood was evaluated over time and during temperature stress. Functional antibodies in dried blood were highly correlated with serum (R2ā€‰=ā€‰0. 98, pā€‰

Concepts Keywords
Blood antibodies
Mab clinical trials
Matched dried blood
Prefusion immunization
Vaccines neutralization
respiratory syncytial virus
serology
vaccine

Semantics

Type Source Name
disease IDO blood
disease VO Respiratory syncytial virus
disease VO storage
disease IDO assay
drug DRUGBANK Palivizumab
disease VO time
disease VO immunization
disease VO vaccine

Original Article

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