A novel three-plasmid packaging system for chimeric SFV/SIN VRPs derived from Semliki Forest virus and Sindbis virus as a candidate gene delivery vector.

A novel three-plasmid packaging system for chimeric SFV/SIN VRPs derived from Semliki Forest virus and Sindbis virus as a candidate gene delivery vector.

Publication date: Jan 01, 2024

Semliki Forest virus (SFV) viral replicon particles (VRPs) have been frequently used in various animal models and clinical trials. Chimeric replicon particles offer different advantages because of their unique biological properties. We here constructed a novel three-plasmid packaging system for chimeric SFV/SIN VRPs. The capsid and envelope of SIN structural proteins were generated using two-helper plasmids separately, and the SFV replicon contained the SFV replicase gene, packaging signal of SIN, subgenomic promoter followed by the exogenous gene, and 3′ UTR of SIN. The chimeric VRPs carried luciferase or eGFP as reporter genes. The fluorescence and electron microscopy results revealed that chimeric VRPs were successfully packaged. The yield of the purified chimeric VRPs was approximately 2. 5 times that of the SFV VRPs (1. 38 cD7 10 TU/ml vs. 5. 41 cD7 10 TU/ml) (p 

Concepts Keywords
Forest Animals
Microscopy chimeric
Models Genetic Vectors
Viral Luciferases
Luciferases
Mice
Plasmids
Replicon
replicon
Semliki forest virus
Semliki Forest virus
Sindbis Virus
Sindbis virus
VRPs

Semantics

Type Source Name
disease VO gene

Original Article

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