A nanogap-enhanced SERS nanotag-based lateral flow assay for ultrasensitive and simultaneous monitoring of SARS-CoV-2 S and NP antigens.

A nanogap-enhanced SERS nanotag-based lateral flow assay for ultrasensitive and simultaneous monitoring of SARS-CoV-2 S and NP antigens.

Publication date: Jan 18, 2024

A lateral flow assay (LFA) strip based on dual 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB)-encoded satellite FeO@Au (Mag@Au) SERS tags with nanogap is reported for ultrasensitive and simultaneous diagnosis of two SARS-CoV-2 functional proteins. Composed of FeO core, satellite gold shell with nanogaps, and double-layer DTNB, the Mag@Au nanoparticles with an average size of 238 nm were designed as multifunctional tags to efficiently enrich the target SARS-CoV-2 protein from complex samples, significantly enhancing the SERS signal of the LFA strip and provide quantitative SERS detection of analyte on test lines. The developed dual DTNB-encoded satellite Mag@Au-based LFA allowed simultaneous quantification of spike (S) protein and nucleocapsid (NP) protein with detection limits of 23 pg mL and 2 pg mL, respectively, lower than commercial ELISA kits and reported SERS-LFA detection system-based Au NPs and FeO@3 nm Au MNPs. This magnetic SERS-LFA also showed high performance of multi-variant strain detection and further distinguished clinical samples of Omicron variant infection, demonstrating the potential of in situ detection of respiratory virus diseases.

Concepts Keywords
Genes Antigen testing
Gold Functional proteins
Nanoparticles Lateral flow assay
Nitrobenzoic Multi-variant strain detection
Target SARS-CoV-2

Semantics

Type Source Name
disease IDO assay
drug DRUGBANK Gold
disease VO SARS-CoV-2 protein
disease MESH infection
disease MESH virus diseases

Original Article

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