Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing.

Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing.

Publication date: Jan 19, 2024

We present an amplicon-based assay for MinION Nanopore sequencing of mpox virus (MPXV) genomes from clinical specimens, obtaining high-quality results with an average genome coverage of 99% for Ct values of up to 25, and a genome coverage of 97. 1% for Ct values from 25 to 30 which are challenging to sequence. This assay is easy to implement in PCR-based workflows and provides accurate genomic data within a short time.

Concepts Keywords
Genomes MinION
Nanopore mpox virus
Pcr Nanopore sequencing
Virus Orthopox viruses

Semantics

Type Source Name
disease IDO assay
disease IDO quality
disease VO time
disease VO Viruses

Original Article

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