Single-Response Duplexing of Electrochemical Label-free Biosensor from the Same Tag.

Single-Response Duplexing of Electrochemical Label-free Biosensor from the Same Tag.

Publication date: Jan 20, 2024

Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy. Exploiting the vertical, meshed design of reproducible and low-cost ultra-dense electrochemical chips, the unprecedented single-response multiplexing of typical label-free biosensors is reported. Using a cheap, handheld one-channel workstation and a single redox probe, i. e., ferro/ferricyanide, the recognition events taking place on two spatially resolved locations of the same working electrode can be tracked along a single voltammetry scan by collecting the electrochemical signatures of the probe in relation to different quasi-reference electrodes, Au (0. 0 V) and Ag/AgCl ink (+0. 2 V). This spatial isolation prevents crosstalk between the redox tags and interferences over functionalization and binding steps, representing an advantage over the existing non-spatially resolved single-response multiplex strategies. As proof of concept, peptide-tethered immunosensors are demonstrated to provide the duplex detection of COVID-19 antibodies from distinct samples, thereby doubling the throughput while achieving 100% accuracy in serum samples. We envision the approach to enable broad applications in high-throughput and multi-analyte platforms, as it can be tailored to other biosensing devices and formats. This article is protected by copyright. All rights reserved.

Concepts Keywords
Biosensing accuracy
Boost Multiplexed detection
Cheap serology
Covid single-channel potentiostat
Ferricyanide square wave voltammetry
steric hindrance


Type Source Name
disease MESH COVID-19

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