Pseudouridine-modified RNA probe for label-free electrochemical detection of nucleic acids on 2D MoS nanosheets.

Pseudouridine-modified RNA probe for label-free electrochemical detection of nucleic acids on 2D MoS nanosheets.

Publication date: Jan 22, 2024

RNA modification, particularly pseudouridine (Ψ), has played an important role in the development of the mRNA-based COVID-19 vaccine. This is because Ψ enhances RNA stability against nuclease activity and decreases the anti-RNA immune response. Ψ also provides structural flexibility to RNA by enhancing base stacking compared with canonical nucleobases. In this report, we demonstrate the first application of pseudouridine-modified RNA as a probe (Ψ-RNA) for label-free nucleic acid biosensing. It is known that MoS has a differential affinity for nucleic acids, which may be translated into a unique electronic signal. Herein, the Ψ-RNA probe interacts with the pristine MoS surface and causes a change in interfacial electrochemical charge transfer in the MoS nanosheets. Compared with an unmodified RNA probe, Ψ-RNA exhibited faster adsorption and higher affinity for MoS. Moreover, Ψ-RNA could bind to complementary RNA and DNA targets with almost equal affinity when engaged with the MoS surface. Ψ-RNA maintained robust interactions with the MoS surface following the hybridization event, perhaps through its extra amino group. The detection sensitivity of the Ψ-RNA/MoS platform was as low as 500 attomoles, while the results also indicate that the probe can distinguish between complementary targets, single mismatches, and non-complementary nucleic acid sequences with statistical significance. This proof-of-concept study shows that the Ψ-RNA probe may solve numerous problems of adsorption-based biosensing platforms due to its stability and structural flexibility.

Concepts Keywords
Biosensing Acids
Mismatches Affinity
Mrna Complementary
Pristine Detection
Vaccine Electrochemical
Free
Label
Modified
Mos
Nanosheets
Nucleic
Probe
Pseudouridine
Rna
Surface

Semantics

Type Source Name
disease VO COVID-19 vaccine
disease IDO immune response
disease VO report
disease MESH causes
drug DRUGBANK Aspartame

Original Article

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