A Portable and Disposable Electrochemical Sensor Utilizing Laser-Scribed Graphene for Rapid SARS-CoV-2 Detection.

A Portable and Disposable Electrochemical Sensor Utilizing Laser-Scribed Graphene for Rapid SARS-CoV-2 Detection.

Publication date: Dec 23, 2023

The COVID-19 pandemic caused by the virus SARS-CoV-2 was the greatest global threat to human health in the last three years. The most widely used methodologies for the diagnosis of COVID-19 are quantitative reverse transcription polymerase chain reaction (RT-qPCR) and rapid antigen tests (RATs). PCR is time-consuming and requires specialized instrumentation operated by skilled personnel. In contrast, RATs can be used in-home or at point-of-care but are less sensitive, leading to a higher rate of false negative results. In this work, we describe the development of a disposable, electrochemical, and laser-scribed graphene-based biosensor strips for COVID-19 detection that exploits a split-ester bond ligase system (termed ‘EsterLigase’) for immobilization of a virus-specific nanobody to maintain the out-of-plane orientation of the probe to ensure the efficacy of the probe-target recognition process. An anti-spike VHH E nanobody, genetically fused with the EsterLigase domain, was used as the specific probe for the spike receptor-binding domain (SP-RBD) protein as the target. The recognition between the two was measured by the change in the charge transfer resistance determined by fitting the electrochemical impedance spectroscopy (EIS) spectra. The developed LSG-based biosensor achieved a linear detection range for the SP-RBD from 150 pM to 15 nM with a sensitivity of 0. 0866 [log(M)] and a limit of detection (LOD) of 7. 68 pM.

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Concepts Keywords
Global Antibodies
Laser Antibodies
Pandemic biosensor
Pcr COVID-19
Rats COVID-19
laser-scribed graphene


Type Source Name
disease MESH COVID-19 pandemic
disease VO time
disease MESH immobilization
disease IDO process
drug DRUGBANK Coenzyme M
drug DRUGBANK Immune Globulin Human
disease MESH infection
disease IDO assay
disease MESH infectious diseases
disease IDO site
disease VO device
disease VO efficient
disease IDO production
drug DRUGBANK Silicon
disease VO effective
drug DRUGBANK Gold
drug DRUGBANK Potassium
drug DRUGBANK Indoleacetic acid
drug DRUGBANK Guanosine
drug DRUGBANK Acetic acid
drug DRUGBANK Phosphate ion
disease VO USA
drug DRUGBANK Butyric Acid
drug DRUGBANK Phenylbutyric acid
disease MESH Influenza
drug DRUGBANK L-Aspartic Acid
disease IDO colony
drug DRUGBANK Ampicillin
drug DRUGBANK Isopropyl beta-D-thiogalactopyranoside
drug DRUGBANK Tromethamine
drug DRUGBANK Imidazole
drug DRUGBANK Edetic Acid
drug DRUGBANK Flunarizine
drug DRUGBANK Sodium lauryl sulfate
drug DRUGBANK L-Lysine
drug DRUGBANK Ethanol
drug DRUGBANK Glycerin
disease VO frequency
drug DRUGBANK Carbonate ion
drug DRUGBANK L-Threonine
disease VO URE
drug DRUGBANK (S)-Des-Me-Ampa
disease MESH Pneumonia
disease MESH Syndrome
drug DRUGBANK Platinum
drug DRUGBANK Estradiol

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