Breath of Health: spectroscopy-based breath test for the detection of SARS-CoV-2.

Publication date: Feb 29, 2024

Nucleic acid amplification tests (NAAT) are considered the gold standard for COVID-19 diagnosis. These tests require professional manpower and equipment, long processing and swab sampling which is unpleasant to the patients. Several volatile organic compounds (VOCs) have been identified in the breath of COVID-19 patients. Detection of these VOCs using a breath test could help rapidly identify COVID-19 patients. Assess the accuracy of ‘Breath of Health’ (BOH) COVID-19 Fourier-transform infra-red (FTIR) Spectroscopy-based breath test. Breath samples from patients with or without symptoms suggestive for COVID-19 who had NAAT results were collected using Tedlar bags and were blindly analysed using BOH FTIR spectroscopy. BOH Measures several VOCs simultaneously and differentiating positive and negative results. BOH results were compared to NAAT results as gold standard. Breath samples from 531 patients were analysed. The sensitivity of BOH breath test was found to be 79. 5% and specificity was 87. 2%. Positive predictive value (PPV) was 74. 7% and negative predictive value (NPV) 90. 0%. Calculated accuracy rate was 84. 8% and area under the curve 0. 834. Subgroup analysis revealed that the NPV of patients without respiratory symptoms was superior over the NPV of symptomatic patients (94. 7% vs 80. 7%, P-value < 0. 0001) and PPV of patients with respiratory symptoms outranks the PPV of individuals without symptoms (85. 3% vs 69. 2%, P-value 0. 0196). We found BOH COVID-19 breath test to be a patient-friendly, rapid, non-invasive diagnostic test with high accuracy rate and NPV that could efficiently rule out COVID-19 especially among individuals with low pre-test probability.

Concepts Keywords
Covid breath test
Nucleic COVID-19
Organic Fourier-transform infra-red spectroscopy
Red SARS-CoV-2


Type Source Name
drug DRUGBANK Gold
disease MESH COVID-19

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