A Wash-Free Spheres-on-Sphere Strategy for On-Site and Multiplexed Biosensing.

Publication date: Mar 07, 2024

Respiratory infections and food contaminants pose severe challenges to global health and the economy. A rapid on-site platform for the simultaneous detection of multiple pathogens is crucial for accurate diagnosis, appropriate treatment, and a reduced healthcare burden. Herein, we present a spheres-on-sphere (SOS) platform for multiplexed detection using a portable Coulter counter, which employs millimeter- and micron-sized spheres coupled with antibodies as multitarget probes. The assay allows for quantitative detection of multiple analytes within 20 min by simple mixing, enabling on-site detection. The platform shows high accuracy in identifying three respiratory viruses (SARS-CoV-2, influenza A virus, and parainfluenza virus) from throat swab samples, with LOD of 50. 7, 32. 4, and 49. 1 pg/mL. It also demonstrates excellent performance in quantifying three mycotoxins (aflatoxin B1, deoxynivalenol, and ochratoxin A) from food samples. The SOS platform offers a rapid on-site approach with high sensitivity and specificity for applications in resource-limited settings.

Concepts Keywords
Biosensing Microspheres
Deoxynivalenol Multiplexed detection
Economy On-site testing
Influenza Respiratory virus
Millimeter Spheres-on-sphere platform


Type Source Name
disease IDO site
disease MESH Respiratory infections
disease IDO assay
disease VO Viruses
drug DRUGBANK Influenza A virus
disease MESH parainfluenza

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