The human MIF polymorphism CATT enhances pro-inflammatory macrophage polarization in a clinically relevant model of allergic airway inflammation.

Publication date: Mar 31, 2024

High level expression of the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) has been associated with severe asthma. The role of MIF and its functional promotor polymorphism in innate immune training is currently unknown. Using novel humanized CATT MIF mice, this study is the first to investigate the effect of MIF on bone marrow-derived macrophage (BMDM) memory after house dust mite (HDM) challenge. CATT BMDMs demonstrated a significant primed increase in M1 markers following HDM and LPS stimulation, compared to naive mice. This M1 signature was found to be MIF-dependent, as administration of a small molecule MIF inhibitor, SCD-19, blocked the induction of this pro-inflammatory M1-like phenotype in BMDMs from CATT mice challenged with HDM. Training naive BMDMs in vitro with HDM for 24 h followed by a rest period and subsequent stimulation with LPS led to significantly increased production of the pro-inflammatory cytokine TNFα in BMDMs from CATT mice but not WT mice. Addition of the pan methyltransferase inhibitor MTA before HDM training significantly abrogated this effect in BMDMs from CATT mice, suggesting that HDM-induced training is associated with epigenetic remodelling. These findings suggest that trained immunity induced by HDM is under genetic control, playing an important role in asthma patients with the high MIF genotypes (CATT).

Concepts Keywords
Asthma Animals
Genetic Asthma
Mice house dust mite
Pro Humans
Remodelling Inflammation
innate immunity
innate priming
innate training
Intramolecular Oxidoreductases
Intramolecular Oxidoreductases
Lipopolysaccharides
Lipopolysaccharides
Macrophage Migration-Inhibitory Factors
Macrophage Migration-Inhibitory Factors
Mice
MIF protein, human
polarization
Pyroglyphidae

Semantics

Type Source Name
disease MESH inflammation
disease MESH asthma
pathway KEGG Asthma
disease VO macrophage
disease IDO production

Original Article

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