Publication date: Jul 13, 2024
Disease diagnostics and surveillance increasingly highlight the importance of portable, cost-effective, and sensitive point-of-care (POC) detection of nucleic acids. Here, we report a CRISPR/Cas13a-responsive and RNA-bridged DNA hydrogel capillary sensor for the direct and visual detection of specific RNA with high sensitivity. The capillary sensor was simply prepared by loading RNA-cross-linking DNA hydrogel film (∼0. 2 mm +/- 0. 02 mm) at the end of a capillary. When CRISPR/Cas13a specifically recognizes the target RNA, the RNA bridge in the hydrogel film is cleaved by the trans-cleavage activity of CRISPR/Cas13a, increasing the permeability of the hydrogel film. Different concentrations of target RNA activate different amounts of Cas13a, cleaving different amounts of the RNA bridge in the hydrogel and causing corresponding changes in the permeability of the hydrogel. Therefore, samples containing different amounts of the target RNA travel to different distances in the capillary. Visual reading of the distance provides quantitative detection of the RNA target without the need for any nucleic acid amplification or auxiliary equipment. The technique was successfully used for the determination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in clinical nasopharyngeal (NP) swab and saliva samples. Easily quantifiable distance using a ruler eliminates the need for any optical or electrochemical detection equipment, making this assay potentially useful for POC and on-site applications.
Concepts | Keywords |
---|---|
Coronavirus | Amounts |
Easily | Bridged |
Hydrogel | Capillary |
Rna | Care |
Surveillance | Cas13a |
Crispr | |
Detection | |
Film | |
Hydrogel | |
Poc | |
Point | |
Responsive | |
Rna | |
Sensor | |
Target |
Semantics
Type | Source | Name |
---|---|---|
disease | VO | effective |
disease | VO | report |
disease | VO | Severe acute respiratory syndrome coronavirus 2 |
disease | IDO | assay |
disease | IDO | site |