Publication date: Jul 15, 2024
Feline coronavirus (FCoV) infection is a leading cause of death in cats. In this study, we produced FCoV-I virus-like particles (VLPs) containing E, M, N, and S proteins using a baculovirus expression system and mixed VLPs with the adjuvants MF59 and CpG 55. 2 to prepare an VLP/MF59/CpG vaccine. After immunization of mice with the vaccine, IgG specific antibodies titers against S and N proteins increased to 1:12,800, and IFN-γ and IL-4 splenocytes were significantly increased. Following immunization of FCoV-negative cats, the S protein antibodies in immunized cats (5/5) increased significantly, with a peak of 1:12,800. Notably, after booster vaccination in FCoV-positive cats, a significant reduction in viral load was observed in the feces of partial cats (4/5), and the FCoV-I negative conversion was found in two immunized cats (2/5). Therefore, the VLP/MF59/CpG vaccine is a promising candidate vaccine to prevent the FCoV infection.
| Concepts | Keywords |
|---|---|
| Antibodies | Feline coronavirus |
| Cats | Immunogenicity |
| Coronavirus | Vaccine |
| Death | Virus-like particles |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | VO | virus-like particle vaccine |
| disease | MESH | infection |
| disease | MESH | cause of death |
| disease | VO | vaccine |
| disease | VO | immunization |
| drug | DRUGBANK | Binetrakin |
| disease | VO | immunized |
| disease | VO | vaccination |