Publication date: Jul 19, 2024
In the search of new inhibitors for human coronavirus (HCoV), we screened extracts of endemic Annonaceae plants on an assay using a cellular model of Huh-7 cells infected with the human alphacoronavirus HCoV-229E. The EtOAc bark extract of the rare Southeast Asian plant Neo-uvaria foetida exhibited inhibition of HCoV-229E and SARS-CoV-2 viruses with IC values of 3. 8 and 7. 8 μg/mL, respectively. Using LC-MS/MS and molecular networking analysis guided isolation, we discovered two new labdane-type diterpenoids, 8-epi-acuminolide (1) and foetidalabdane A (4), and three known labdane diterpenoids, acuminolide (2), 17-O-acetylacuminolide (3), and spiroacuminolide (5). A new norlabdane diterpene, 16-foetinorlabdoic acid (6), was also isolated and identified. Excluding compounds 5 and 6, all other metabolites were active against the virus HCoV-229E. Terpenoids 1 and 4 presented antiviral activity against SARS-CoV-2 with IC values of 63. 3 and 93. 5 μM, respectively, indicating lower potency. Additionally, virological assays demonstrated that compounds 1, 2, and 3 exert antiviral effects against Zika virus by specifically interfering with the late stage of its infectious cycle with IC values of 76. 0, 31. 9, and 14. 9 μM, respectively.
| Concepts | Keywords |
|---|---|
| Acetylacuminolide | 229e |
| Alphacoronavirus | Annonaceae |
| Bark | Antiviral |
| Molecular | Bark |
| Rare | Foetida |
| Hcov | |
| Isolation | |
| Labdane | |
| Lc | |
| Molecular | |
| Ms | |
| Neo | |
| Networking | |
| Uvaria | |
| Values |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | IDO | assay |
| disease | VO | Viruses |