Peptide nucleic acid probe-assisted paper-based electrochemical biosensor for multiplexed detection of respiratory viruses.

Publication date: Nov 01, 2024

The similar transmission patterns and early symptoms of respiratory viral infections, particularly severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza (H1N1), and respiratory syncytial virus (RSV), pose substantial challenges in the diagnosis, therapeutic management, and handling of these infectious diseases. Multiplexed point-of-care testing for detection is urgently needed for prompt and efficient disease management. Here, we introduce an electrochemical paper-based analytical device (ePAD) platform for multiplexed and label-free detection of SARS-CoV-2, H1N1, and RSV infection using immobilized pyrrolidinyl peptide nucleic acid probes. Hybridization between the probes and viral nucleic acid targets causes changes in the electrochemical response. The resulting sensor offers high sensitivity and low detection limits of 0. 12, 0. 35, and 0. 36 pM for SARS-CoV-2 (N gene), H1N1, and RSV, respectively, without showing any cross-reactivities. The amplification-free detection of extracted RNA from 42 nasopharyngeal swab samples was successfully demonstrated and validated against reverse-transcription polymerase chain reaction (range of cycle threshold values: 17. 43-25. 89). The proposed platform showed excellent clinical sensitivity (100 %) and specificity (≥97 %) to achieve excellent agreement (_705 ≥ 0. 914) with the standard assay, thereby demonstrating its applicability for the screening and diagnosis of these respiratory diseases.

Concepts Keywords
Biosensor Amperometry
Coronavirus Biosensing Techniques
Efficient COVID-19
Pyrrolidinyl Electrochemical detection
Electrochemical Techniques
Humans
Influenza, Human
Label-free detection
Limit of Detection
Multiplexed detection
Paper
Paper-based analytical device
Peptide Nucleic Acids
Peptide Nucleic Acids
Respiratory Syncytial Viruses
RNA, Viral
RNA, Viral
SARS-CoV-2
Swab samples

Semantics

Type Source Name
disease VO Viruses
disease MESH viral infections
disease VO Severe acute respiratory syndrome coronavirus 2
disease MESH influenza
disease VO Respiratory syncytial virus
disease MESH infectious diseases
disease VO efficient
disease VO device
disease MESH infection
disease MESH causes
disease VO gene
disease IDO assay
disease MESH respiratory diseases
disease MESH COVID-19
drug DRUGBANK Influenza A virus
disease VO H1N1 subtype
disease MESH Respiratory Syncytial Virus Infections

Original Article

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