Publication date: Oct 10, 2024
The detection of diverse pathogens and chemical compounds in community wastewater facilitates the monitoring of public health trends of emerging diseases and health conditions. During the COVID-19 pandemic, detection of viral RNA in wastewater assisted in monitoring the infection rates in different geographies and this drew attention to the method of wastewater-based epidemiology (WBE). In contrast to individual clinical testing, WBE offers an affordable, population-wide overview of the infection status of a community including asymptomatic carriers and those without access to healthcare facilities. To understand the community status of the infections caused by respiratory syncytial virus, SARS-CoV-2 and influenza virus subtypes in the monsoon season in Vijayawada city in the state of Andhra Pradesh in India, we longitudinally analyzed wastewater samples once a week between July-August 2024 from 7 sewage treatment plants in the city. The data obtained from the multiplexed RT-qPCR was used to calculate the viral loads in the wastewater samples at the city level and the proportion of population shedding the virus was estimated. All three viruses RSV A+B, SARS-CoV-2 and Influenza A+B were detected in the wastewater during our sampling period. Amongst the three viruses, the city had the largest load of SARS-CoV-2 RNA in the wastewater followed by RSV A+B and Influenza A+B. The study demonstrates the potential of utilizing wastewater surveillance system coupled to multiplexed RT-PCR to understand the population level dynamics of co-existence of multiple pathogens during the monsoon season in the urban settings.
| Concepts | Keywords |
|---|---|
| Biosafety | Al |
| July | City |
| Poliovirus | Cov |
| Influenza | |
| Location | |
| Medrxiv | |
| Preprint | |
| Respiratory | |
| Rsv | |
| Sampling | |
| Sars | |
| Viral | |
| Virus | |
| Viruses | |
| Wastewater |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | influenza |
| disease | MESH | COVID-19 pandemic |
| disease | MESH | infection |
| pathway | KEGG | Influenza A |
| disease | MESH | syndrome |
| disease | MESH | parainfluenza |
| disease | MESH | respiratory infections |
| disease | MESH | viral shedding |
| disease | MESH | infectious diseases |
| drug | DRUGBANK | 4-Methyl-2 5-dimethoxyamphetamine |
| drug | DRUGBANK | Polyethylene glycol |
| drug | DRUGBANK | Sodium Chloride |
| drug | DRUGBANK | Flunarizine |
| disease | MESH | viral load |
| disease | MESH | avian influenza |
| disease | IDO | pathogen |
| disease | MESH | hepatitis |
| drug | DRUGBANK | Spectinomycin |
| disease | MESH | Enterovirus Infections |
| disease | MESH | Co infection |
| disease | MESH | clinical significance |
| disease | IDO | pathogen surveillance |
| drug | DRUGBANK | Nonoxynol-9 |