Publication date: Nov 16, 2024
Multiple respiratory viruses lead to high morbidity and mortality, yet global surveillance platforms focus primarily on seasonal influenza viruses. The COVID-19 pandemic and new RSV vaccines highlight the importance of a broader approach. Upper respiratory tract swabs from children aged 24-59 months presenting with influenza-like illness in The Gambia were collected during follow-up of a live-attenuated influenza vaccine randomised controlled trial in 2017-18. A microfluidic quantitative polymerase chain reaction (qPCR) assay was established and used to detect 21 respiratory viruses. 76. 6% of samples had one or more viruses detected (n = 121/158). The viruses detected most frequently were rhinovirus (n = 37/158, 23. 4%) and adenovirus (n = 34/158, 21. 5%), followed by parainfluenza virus 3, influenza B and human metapneumovirus B. A third of positive samples had multiple viruses detected (two n = 31/121, 25. 6%; three n = 9/121, 7. 4%). Our data demonstrates how microfluidic qPCR is a useful tool for high-throughput, comprehensive detection of multiple respiratory viruses in surveillance platforms. Rapidly changing epidemiology exemplifies the need for new, broader approaches to virus surveillance in low-resource settings to respond to future epidemics and to guide the need for and use of new prevention and therapeutic measures.
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Semantics
Type | Source | Name |
---|---|---|
disease | MESH | influenza |
disease | MESH | morbidity |
disease | MESH | COVID-19 pandemic |
disease | IDO | assay |
disease | MESH | parainfluenza |
disease | MESH | respiratory tract infections |
disease | IDO | reagent |
disease | MESH | Infection |
drug | DRUGBANK | Coenzyme M |
disease | IDO | history |
disease | MESH | sore throat |
drug | DRUGBANK | Methionine |
disease | IDO | nucleic acid |
disease | IDO | pathogen |
drug | DRUGBANK | Water |
disease | IDO | process |
disease | IDO | replication |
drug | DRUGBANK | Saquinavir |
disease | IDO | production |
disease | IDO | ribonucleic acid |
disease | MESH | co infection |
disease | IDO | symptom |
disease | MESH | Middle East Respiratory Syndrome |
disease | IDO | virulence |
disease | MESH | etiology |
drug | DRUGBANK | Adenosine |
disease | MESH | Respiratory syncytial virus infection |
pathway | REACTOME | Release |
drug | DRUGBANK | Etoperidone |
pathway | REACTOME | Reproduction |
drug | DRUGBANK | Influenza B virus |