Microfluidic qPCR for detection of 21 common respiratory viruses in children with influenza-like illness.

Publication date: Nov 16, 2024

Multiple respiratory viruses lead to high morbidity and mortality, yet global surveillance platforms focus primarily on seasonal influenza viruses. The COVID-19 pandemic and new RSV vaccines highlight the importance of a broader approach. Upper respiratory tract swabs from children aged 24-59 months presenting with influenza-like illness in The Gambia were collected during follow-up of a live-attenuated influenza vaccine randomised controlled trial in 2017-18. A microfluidic quantitative polymerase chain reaction (qPCR) assay was established and used to detect 21 respiratory viruses. 76. 6% of samples had one or more viruses detected (n = 121/158). The viruses detected most frequently were rhinovirus (n = 37/158, 23. 4%) and adenovirus (n = 34/158, 21. 5%), followed by parainfluenza virus 3, influenza B and human metapneumovirus B. A third of positive samples had multiple viruses detected (two n = 31/121, 25. 6%; three n = 9/121, 7. 4%). Our data demonstrates how microfluidic qPCR is a useful tool for high-throughput, comprehensive detection of multiple respiratory viruses in surveillance platforms. Rapidly changing epidemiology exemplifies the need for new, broader approaches to virus surveillance in low-resource settings to respond to future epidemics and to guide the need for and use of new prevention and therapeutic measures.

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Concepts Keywords
Global Adenoviridae
Pandemic Child, Preschool
Rhinovirus Female
Therapeutic Humans
Infant
Influenza B virus
Influenza-like illness
Influenza, Human
Male
Microfluidics
Microfluidics
Parainfluenza Virus 3, Human
Polymerase chain reaction
Respiratory Tract Infections
Respiratory virus
Rhinovirus
Surveillance
Viruses

Semantics

Type Source Name
disease MESH influenza
disease MESH morbidity
disease MESH COVID-19 pandemic
disease IDO assay
disease MESH parainfluenza
disease MESH respiratory tract infections
disease IDO reagent
disease MESH Infection
drug DRUGBANK Coenzyme M
disease IDO history
disease MESH sore throat
drug DRUGBANK Methionine
disease IDO nucleic acid
disease IDO pathogen
drug DRUGBANK Water
disease IDO process
disease IDO replication
drug DRUGBANK Saquinavir
disease IDO production
disease IDO ribonucleic acid
disease MESH co infection
disease IDO symptom
disease MESH Middle East Respiratory Syndrome
disease IDO virulence
disease MESH etiology
drug DRUGBANK Adenosine
disease MESH Respiratory syncytial virus infection
pathway REACTOME Release
drug DRUGBANK Etoperidone
pathway REACTOME Reproduction
drug DRUGBANK Influenza B virus

Original Article

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