High-throughput specificity profiling of antibody libraries using ribosome display and microfluidics.

High-throughput specificity profiling of antibody libraries using ribosome display and microfluidics.

Publication date: Dec 16, 2024

In this work, we developed PolyMap (polyclonal mapping), a high-throughput method for mapping protein-protein interactions. We demonstrated the mapping of thousands of antigen-antibody interactions between diverse antibody libraries isolated from convalescent and vaccinated COVID-19 donors and a set of clinically relevant SARS-CoV-2 spike variants. We identified over 150 antibodies with a variety of distinctive binding patterns toward the antigen variants and found a broader binding profile, including targeting of the Omicron variant, in the antibody repertoires of more recent donors. We then used these data to select mixtures of a small number of clones with complementary reactivity that together provide strong potency and broad neutralization. PolyMap is a generalizable platform that can be used for one-pot epitope mapping, immune repertoire profiling, and therapeutic design and, in the future, could be expanded to other families of interacting proteins.

Concepts Keywords
Antibody Antibodies, Neutralizing
Future Antibodies, Neutralizing
Libraries Antibodies, Viral
Microfluidics Antibodies, Viral
Thousands Antibody Specificity
antibody-antigen interaction
cell surface protein
COVID-19
CP: Biotechnology
CP: Immunology
Epitope Mapping
high-throughput screening
High-Throughput Screening Assays
Humans
Microfluidics
Peptide Library
Peptide Library
polyclonal antibody profiling
PolyMap
protein-protein interaction
ribosome display
Ribosomes
SARS-CoV-2
SARS-CoV-2 surface antigen
single-cell analysis
Spike Glycoprotein, Coronavirus
Spike Glycoprotein, Coronavirus
spike protein, SARS-CoV-2
viral neutralization

Semantics

Type Source Name
pathway KEGG Ribosome
disease IDO protein
disease MESH COVID-19
disease IDO cell

Original Article

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