Publication date: Jan 21, 2025
Immunoglobulin (Ig) A acts as a first line of defense against respiratory pathogens. Mucosal IgA in salivary and nasal passages has a rapid response to antigens and can play a protective role against reinfection. The mainstay for analyzing SARS-CoV-2 infection and vaccine efficacy has been assessment of serum IgG levels; however, validated assays for assessment of mucosal IgA in clinical samples are necessary as new and adapted measures are generated to combat immune-evasive viral variants. A mucosal IgA assay was developed and tested through assessment of IgA levels in salivary samples from participants of the 2019nCoV-314/ NCT05973006 study. These participants had previously received [≥]2 mRNA-based COVID-19 vaccinations prior to enrollment and received a single intramuscular study dose of NVX-CoV2601 (XBB.1.5) or bivalent vaccine (NVX-CoV2601 + NVX-CoV2373 [Wuhan]). Salivary samples were collected prior to vaccination on day 0 and on day 28 to assess response post vaccination. Both vaccine groups elicited a significant increase in anti -SARS-CoV-2 spike IgA against XBB.1.5. Furthermore, cross-reactivity via identification of anti-JN.1 and anti-Wuhan IgA was also observed. The detection of IgA in clinical mucosal samples through this assay will be a valuable tool in supporting vaccine development.
| Concepts | Keywords |
|---|---|
| Aguilera | Clinical |
| Boykins | Cov |
| Immunoglobulin | Covid |
| Influenza | Https |
| Iga | |
| Medrxiv | |
| Mucosal | |
| Novavax | |
| Nvx | |
| Preprint | |
| Salivary | |
| Sars | |
| Spike | |
| Vaccine | |
| Xbb |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | IDO | role |
| disease | MESH | reinfection |
| disease | MESH | SARS-CoV-2 infection |
| pathway | REACTOME | SARS-CoV-2 Infection |
| disease | IDO | assay |
| drug | DRUGBANK | Coenzyme M |
| disease | IDO | production |
| disease | IDO | susceptibility |
| disease | MESH | infection |
| disease | MESH | recurrence |
| disease | IDO | symptom |
| disease | MESH | breakthrough infections |
| disease | MESH | influenza |
| disease | MESH | infection transmission |
| disease | IDO | pathogen |
| disease | MESH | comorbidity |
| disease | MESH | clinical significance |
| disease | MESH | Allergy |
| disease | MESH | IgA Deficiency |
| disease | MESH | viral infection |
| disease | MESH | severe acute respiratory syndrome |
| disease | MESH | pneumonia |
| disease | MESH | Infectious Diseases |
| disease | IDO | blood |