Publication date: Jan 18, 2025
The signature of human serum IgG glycosylation is critical in the defense against pathogens. Alterations of IgG N-glycome were associated with COVID-19 (Coronavirus disease 2019) severity, although knowledge on the response to vaccination is limited. IgG N-glycome was analyzed in this study in post-COVID-19 and post-vaccination patients to reveal potential glycosylation-based alterations using hydrophilic interaction liquid chromatography (HILIC-UPLC) with fluorescence (FLR) and mass-spectrometric (MS) detection. IgG antibodies were purified from serum samples through protein G affinity chromatography followed by PNGase F digestion-based deglycosylation. The released glycans were fluorescently derivatized by procainamide labeling and purified via solid-phase extraction. Higher levels of sialylation and afucosylation were identified in post-COVID-19 patients, which was further expanded by vaccination, but only in those who were previously SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) infected.
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Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | COVID-19 |
| disease | IDO | protein |
| pathway | REACTOME | Digestion |
| drug | DRUGBANK | Procainamide |
| disease | IDO | blood |
| pathway | REACTOME | Immune System |
| disease | MESH | viral infections |
| disease | IDO | production |
| drug | DRUGBANK | L-Asparagine |
| disease | MESH | infection |
| drug | DRUGBANK | Coenzyme M |
| drug | DRUGBANK | Hexocyclium |
| drug | DRUGBANK | Formic Acid |
| drug | DRUGBANK | Acetic acid |
| drug | DRUGBANK | Dimethyl sulfoxide |
| drug | DRUGBANK | Water |
| drug | DRUGBANK | Flunarizine |
| disease | MESH | Allergy |
| disease | MESH | bacterial diseases |
| disease | IDO | intervention |