Publication date: Feb 03, 2025

MXenes are a growing family of two-dimensional (2D) layered transition metal carbides, nitrides, and/or carbonitrides. Recently, these materials have been used in many sensing and biosensing platforms because of their excellent electrochemical characteristics. In this work, we investigate the applicability of double transition metal (DTM)-based MXenes in the electrochemical immunosensing of the respiratory syncytial virus (RSV). This ubiquitous virus is considered a major pathogen causing acute lower respiratory tract infections in young children and elderly individuals. The immunosensor was constructed by immobilizing the RSV antibody on screen-printed carbon electrodes modified with graphene oxide and the MoTiCT DTM MXene. The presence of the RSV antigen was detected in a label-free mode using square wave voltammetry. A low limit of detection of 0. 015 pg mL and a remarkable selectivity against other bacterial and viral pathogens, including coronavirus, were achieved. We also compared this MXene with the standard TiCT and confirmed that it has a 1. 21-fold higher electrochemically active effective surface area. The applicability of the MoTiCT MXene-based immunosensor in real serum samples was also investigated, yielding excellent recovery percentages ranging from 95. 48 to 98. 59%.

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