Publication date: Feb 10, 2025
A novel method was proposed based on gold immune chromatography assay (GICA) including the detection of antibodies targeting different severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epitopes to improve the SARS-CoV-2 IgM and IgG detection performance. Sera from 282 confirmed Coronavirus Disease 2019 (COVID-19) patients were obtained at different times as the SARS-CoV-2 IgM or IgG experimental group. Sera from 148 uninfected and unvaccinated individuals were used as the control. The serum single-epitope IgM and IgG antibodies against SARS-CoV-2 were detected via GICA; the two epitope antibodies with high detection performance were used to construct a novel method, and then compared with the chemiluminescence immunoassay (CLIA). The diagnostic specificity and screening sensitivity of S2-IgM and N-IgM combined detection of serum SARS-CoV-2 IgM antibodies based on GICA were 99. 32% and 98. 81%, respectively, which were higher than those of the CLIA test (83. 78% and 82. 14%; P
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| Concepts | Keywords |
|---|---|
| Antibodies | Chemiluminescence Immunoassay |
| Chromatography | Diagnostic specificity |
| Coronavirus | SARS-CoV-2 IgG antibody |
| Covid | SARS-CoV-2 IgM antibody |
| Screening sensitivity |
Semantics
| Type | Source | Name |
|---|---|---|
| drug | DRUGBANK | Gold |
| disease | IDO | assay |
| disease | MESH | Coronavirus Disease 2019 |