Publication date: Feb 10, 2025

COVID-19 disease has led to many deaths worldwide and early detection of people at a high risk of severe forms of this disease would greatly help physicians. The presence of oxidative stress biomarkers may help identify high-risk individuals early in the course of the disease. 8-Hydroxy-2′-deoxyguanosine (8-OHdG) is a widely used biomarker for assessing endogenous oxidative DNA damage. In this study, the urinary 8-OHdG levels were determined in COVID-19 patients and COVID-19 patients with cancer by a dispersive liquid-liquid microextraction (DLLME) method using gas chromatography-mass spectrometry (GC-MS). The effects of essential parameters on the extraction method were investigated. The LOD and LOQ are equal to 1. 7 nM and 5. 1 nM, respectively. At varied concentrations of 8-OHdG (300, 400, and 600 nM), the relative standard deviation (RSD) ranged from 18. 35% to 22. 36%. The mean urinary 8-OHdG levels of cancer and COVID-19 patients were 13. 20 +/- 6. 20 nmol mmol, while the mean levels in COVID-19 patients and healthy volunteers were 6. 67 +/- 5. 80 nmol mmol and 1. 61 +/- 1. 72 nmol mmol, respectively. The results of this study showed that the level of 8-OHdG urine biomarkers in people with COVID-19 is significantly higher than in healthy people. In this study, the DLLME approach was used for the first time to determine the value of 8-OHdG using GC-MS. According to the results of this research, the DLLME method was successfully used as a biomarker of DNA oxidative stress for extracting 8-OHdG urine. Compared to other methods, this technique has advantages such as shorter extraction time and low cost.

Semantics

Original Article