Publication date: Feb 12, 2025
The application is demonstrated of injection-molded centrifugal microfluidic chips with integrated optical pH sensors for multiplexed detection of respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A, and influenza B RNA. The optical pH sensors generated sensitive fluorescent readouts from diagnostic reverse transcription loop-mediated isothermal amplification (RT-LAMP) reactions; limits of detection for influenzas A and B, and SARS-CoV-2 of 89, 245, and 38 RNA copies per reaction, respectively, were attained. Results were obtainable within 44 min for SARS-CoV-2 and influenza A, and 48 min for influenza B. We implemented a data processing strategy based on numerical derivatives of the fluorescence curves that allowed for reliable, quantitative thresholds for deciding reaction outcomes and enabled 100% specificity. This work demonstrates the utility of optical pH sensors and injection-molded centrifugal microfluidics for multiplexed infectious disease diagnostics with point-of-care applications.
Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | syndrome |
| pathway | KEGG | Influenza A |
| disease | MESH | influenza |
| disease | MESH | infectious disease |
| pathway | REACTOME | Infectious disease |
| disease | MESH | COVID-19 |
| drug | DRUGBANK | Influenza A virus |
| drug | DRUGBANK | Influenza B virus |
| disease | IDO | nucleic acid |