Publication date: May 25, 2025

Wastewater can be a possible source of virus transmission. Therefore, the present research evaluates the presence of SARS-CoV-2 RNA in untreated municipal wastewater in Behbahan County, Iran. 24 wastewater samples were collected from January to February 2021. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied for the detection of RNA of the virus of interest. Based on the results, SARS-CoV-2 RNA was detected with concentrations ranging from 0 to 2100 gene copies/L in studied samples. A strong positive correlation (r = 0. 76, p 

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