Publication date: Jul 11, 2025
This study aimed to generate a monoclonal antibody (mAb) against feline coronavirus (FCoV) spike (S) protein and to develop a colloidal gold immunochromatographic strip for the rapid and accurate FCoV detection. BALB/c mice were immunized with the purified protein, and hybridoma technology was employed to produce highly effective mAb. A positive hybridoma cell line (E5) was identified, which stably secreted mAb with a high titer of 1:256000 against the FCoV S recombinant protein. Western blot analysis confirmed the mAb E5’s specificity. The established test strip can detect FCoV specifically and detect the antigen concentrations as low as 1. 2 cD7 10 mg/mL. The diagnostic sensitivity and specificity of the FCoV detection strip for feline coronavirus infections were 94. 2% and 100%, respectively, as confirmed by reverse transcription polymerase chain reaction (RT-PCR). The detection strip demonstrated no cross-reactivity with other feline pathogens and showed consistent results in reproducibility tests. The developed colloidal gold test strip offers a highly sensitive and specific tool for rapid FCoV detection, contributing to improved real-time epidemic monitoring.
| Concepts | Keywords |
|---|---|
| Colloidal | colloidal gold |
| Immunochromatographic | feline coronavirus |
| Viral | immunochromatographic strip |
| monoclonal antibody | |
| prokaryotic expression |
Semantics
| Type | Source | Name |
|---|---|---|
| drug | DRUGBANK | Gold |
| disease | IDO | protein |
| disease | IDO | cell |