Publication date: Sep 10, 2025
Bacteriophage MS2 virus-like particles (VLPs) represent a promising platform for biomedical applications. However, challenges remain in integrating protein display, RNA encapsulation, and convenient purification methods. Here, we developed a universal single-plasmid single-promoter system (USS) that enables modular assembly of multifunctional VLPs through designated PstI (protein insertion) and KpnI (RNA encapsulation) sites. The USS system achieved: 1) Efficient display of large protein molecules (e. g., EGFP, Nanobody N16) on each VLP surface while encapsulating target RNA; 2) Rapid one-step purification via His-tag affinity chromatography, bypassing ultracentrifugation; 3) The resulting VLPs exhibit thermal stability (65^0C for 15minutes) and nuclease resistance. Functional validation demonstrated: 1) Diagnostics: CoviN-encapsulated VLPs served as RT-qPCR controls for COVID-19 detection (linear range: 10-10 copies/μL); 2) Dual functionality for co-delivery: N16-VLPs with shPCSK9 suppressed protein PCSK9 expression by 68% (p
| Concepts | Keywords |
|---|---|
| Biotechnol | MS2 virus-like particles |
| Chromatography | Phage display |
| Efficient | Protein-RNA co-delivery |
| Ms2 | Therapeutic RNA |
| Virus |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | IDO | protein |
| disease | MESH | COVID-19 |
| disease | IDO | production |