Publication date: Sep 12, 2025
Nucleic acid amplification tests (NAATs) are powerful medical diagnostic tools for point-of-care (POC) and other field applications. However, traditional methods like quantitative PCR (qPCR) require complex, expensive equipment and trained operators, limiting their use to centralized labs. Isothermal alternatives, like Loop-mediated Isothermal Amplification (LAMP), are better adapted for POC devices. Lab-on-PCB systems have the potential to overcome the challenges faced by conventional microfabrication-based systems. This study presents a novel lab-on-PCB device for nucleic acid amplification and electrochemical detection using reverse transcription LAMP (RT-LAMP) of SARS-CoV-2. The system consists of two disposable PCB-based chips making it close to zero cost. One PCB is for heating and nucleic acid amplification, while the other is for electrochemical detection using Cyclic Voltammetry (CV) with a redox-active intercalator. The PCB slides are connected to a compact electronic device (
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| Concepts | Keywords |
|---|---|
| Electroanalytical | Electrochemical biosensor. |
| Expensive | Lab-on-PCB. |
| Isothermal | Point-of-care. |
| Lab | |
| Pcr |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | IDO | nucleic acid |
| drug | DRUGBANK | Tropicamide |