Publication date: Jan 01, 2026
Porcine epidemic diarrhea virus (PEDV) induces acute enteric disease in swine, finally leading to neonatal piglets dehydration to death, and which has a great side effect on China’s pig-breeding industry. Therefore, it is urgent to find an economical and effective treatment to prevent and control PEDV. In this study, we identified a high-affinity peptide P6 that specifically targets the C-terminal domain (CTD) of PEDV S1 protein. Subsequently, peptide P6 was conjugated to poly (lactic-co-glycolic acid) (PLGA) via dehydration synthesis to generate PLGA-P6 nanoparticles. The antiviral effect of PLGA-P6 nanoparticles was evaluated through cell counting kit-8 (CCK-8), real-time fluorescence quantitative PCR (qRT-PCR), Western blot and indirect immunofluorescence (IFA). Results showed that peptide P6 exhibited high binding affinity to PEDV S1 protein, among which hydrogen and electrostatic interactions are the key between peptide P6 and PEDV S1 active pocket. Toxicity test suggested that cell viability was >95 % when treated with PLGA-P6 nanoparticles at concentrations not exceeding 1000 μg/mL. Furthermore, absolute quantitative PCR demonstrated that 400 μg/mL PLGA-P6 nanoparticles significantly reduce viral load of PEDV compared to the virus group (P
Semantics
| Type | Source | Name |
|---|---|---|
| drug | DRUGBANK | Glycolic acid |
| disease | MESH | diarrhea |
| disease | MESH | virus infection |
| disease | MESH | dehydration |
| disease | MESH | death |
| drug | DRUGBANK | Sincalide |
| disease | MESH | Coronavirus Infections |
| disease | MESH | Swine Diseases |