Publication date: Jan 01, 2026
Porcine epidemic diarrhea virus (PEDV), a highly infectious porcine virus, leads to high mortality in newborn piglets. Bile acids (BAs) are synthesized in the liver and metabolized in the intestines by gut microbiota. These acids can regulate cellular immunity and influence viral infection processes. To assess the antiviral activity of four BAs-cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), and lithocholic acid (LCA) against PEDV in IPEC-J2 cells. We investigated the effects of 24-h pretreatment with the four aforementioned BAs at varying concentrations on PEDV replication in IPEC-J2 cells through quantitative polymerase chain reaction (qPCR), Western blotting, and TCID assay. BA exposure inhibited PEDV N protein expression to varying degrees. qPCR and Western blotting to measure PEDV N protein and mRNA expression at varying LCA concentrations (1, 5, or 10 μM) revealed that LCA demonstrated significant anti-PEDV activity in a concentration-dependent manner (p
Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | diarrhea |
| disease | MESH | viral infection |
| drug | DRUGBANK | Cholic Acid |
| drug | DRUGBANK | Chenodeoxycholic acid |
| drug | DRUGBANK | Deoxycholic Acid |
| drug | DRUGBANK | Dichloroacetic Acid |
| disease | MESH | LCA |
| disease | MESH | Coronavirus Infections |
| disease | MESH | Swine Diseases |