Airborne transmission-mediated delivery of a live-attenuated influenza virus vector-based intranasal COVID-19 vaccine protects Syrian hamsters against SARS-CoV-2 challenge.

Airborne transmission-mediated delivery of a live-attenuated influenza virus vector-based intranasal COVID-19 vaccine protects Syrian hamsters against SARS-CoV-2 challenge.

Publication date: Dec 12, 2025

Given their critical role in eliciting respiratory immunity, mucosal SARS-CoV-2 vaccines receive significant research attention. Our previous phase 1/2 trial revealed that aerosolized live-attenuated vectored influenza virus COVID-19 vaccine likely elicited specific cellular immunity and weak s-IgA responses in placebo recipients. Here, we demonstrated that viral vector vaccines undergo contact or airborne transmission in various animal models. Vaccines have difficulty spreading in the mouse model, but can effectively spread through the air in more sensitive ferrets. However, the virus transmission in ferrets can be effectively restricted by ventilation system. Furthermore, contact transmission of virus vaccine provided effective protection against lung pathology post-challenge with SARS-CoV-2 in hamsters. These findings suggest the potential to achieve herd immunity in specialized scenarios through airborne or contact transmission of dNS1-RBD, provided that appropriate delivery and control measures are implemented during the administration of live attenuated or viral vectored vaccines.

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Concepts Keywords
Herd Airborne
Models Attenuated
Mucosal Contact
Pathology Cov
Vaccines Covid
Delivery
Hamsters
Immunity
Influenza
Sars
Transmission
Vaccine
Vaccines
Vector
Virus

Semantics

Type Source Name
disease MESH influenza
drug DRUGBANK Medical air
pathway REACTOME Reproduction
disease MESH included
disease MESH Infectious Diseases
disease MESH COVID 19
disease MESH emergency
disease MESH infection
disease MESH Ad5
disease MESH NS1
disease MESH weight loss
disease MESH strains
disease MESH upper respiratory tract infection
disease MESH body weight change
disease MESH inflammation
disease MESH chronic diseases
disease MESH genome stability
drug DRUGBANK Isoxaflutole
pathway REACTOME Immune System
drug DRUGBANK Spinosad
drug DRUGBANK Dextrose unspecified form
disease MESH FBS
drug DRUGBANK Streptomycin
drug DRUGBANK Methylergometrine
drug DRUGBANK Trypsin
disease MESH PBS
drug DRUGBANK Formaldehyde
drug DRUGBANK Gentian violet cation
drug DRUGBANK Amino acids
disease MESH Emerging Infectious Diseases
drug DRUGBANK Isoflurane
drug DRUGBANK Ketamine
drug DRUGBANK Xylazine
drug DRUGBANK Cefaclor
disease MESH CCL
disease MESH fed
disease MESH body weight
drug DRUGBANK Edetic Acid
disease MESH Hemorrhage
disease MESH pulmonary edema
drug DRUGBANK Coenzyme M
pathway REACTOME Signal Transduction
disease MESH Varicella
disease MESH Zoster
disease MESH polio
disease MESH Image
drug DRUGBANK Proline
disease MESH BCD

Original Article

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