Publication date: Dec 12, 2025
Broadly neutralizing monoclonal antibodies (bnAbs) against SARS-CoV-2 are crucial for developing therapeutics and vaccines that confer broad protection against SARS-CoV-2 variants and related sarbecoviruses. Here, we present a protocol to isolate bnAbs against SARS-CoV-2 from human B cells. We describe steps for sorting single spike-specific B cells from human peripheral blood mononuclear cells, cloning monoclonal antibodies (mAbs) from single B cells, screening mAbs with broadly neutralizing activity, and measuring the neutralizing potency of bnAbs against a panel of pseudoviruses. For complete details on the use and execution of this protocol, please refer to Chen et al. .
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| Concepts | Keywords |
|---|---|
| Antibodies | Antibody |
| Complete | High-throughput screening |
| Execution | Immunology |
| Sarbecoviruses | Microbiology |
| Therapeutics | Molecular biology |
| Single cell |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | STAR |
| disease | MESH | infection |
| drug | DRUGBANK | Edetic Acid |
| disease | MESH | PBS |
| disease | MESH | FBS |
| drug | DRUGBANK | Coenzyme M |
| drug | DRUGBANK | Nitrogen |
| drug | DRUGBANK | Glycerin |
| drug | DRUGBANK | Ampicillin |
| drug | DRUGBANK | Methylergometrine |
| disease | MESH | SMS |
| drug | DRUGBANK | Sodium lauryl sulfate |
| disease | MESH | SDS |
| drug | DRUGBANK | Water |
| drug | DRUGBANK | 2′-deoxyuridylic acid |
| disease | MESH | APC |
| drug | DRUGBANK | Immune Globulin Human |
| disease | MESH | strains |
| drug | DRUGBANK | Glycine |
| drug | DRUGBANK | Phosphate ion |
| drug | DRUGBANK | Trypsin |
| drug | DRUGBANK | Streptomycin |
| drug | DRUGBANK | Ethanol |
| drug | DRUGBANK | Sodium Chloride |
| drug | DRUGBANK | Tromethamine |
| drug | DRUGBANK | Fosfomycin |
| disease | MESH | FCM |
| disease | MESH | Rad |
| drug | DRUGBANK | Dextrose unspecified form |
| disease | MESH | ddH |
| disease | MESH | rupture |
| drug | DRUGBANK | Aspartame |
| drug | DRUGBANK | Medical air |
| pathway | REACTOME | Digestion |
| disease | MESH | shock |
| drug | DRUGBANK | Esomeprazole |
| disease | MESH | Plan |
| drug | DRUGBANK | Hepatitis B Vaccine (Recombinant) |
Original Article
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