Publication date: Dec 22, 2025
BACKGROUNDPlatelets are increasingly recognized as active participants in immune signaling and systemic inflammation. Upon activation, platelets form monocyte platelet aggregates (MPA) representing the crossroads of thrombosis and inflammation. We hypothesized that platelet transcriptomics could capture this thromboinflammatory axis and identify individuals at elevated cardiovascular risk. METHODS: MPA levels, defined as CD14+CD61+ cells, were measured using flow cytometry at 2 time points, 4 weeks apart, in healthy individualsPlatelets were isolated and sequenced. Individuals were categorized as MPAhi or MPAlo based on consistently high or low MPA levels across time points. RESULTSAmong 149 participants (median age 52 years, 57% female, 50% non-White), MPAhi individuals exhibited increased expression of platelet activation markers P-selectin (P
Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | thromboinflammation |
| disease | MESH | inflammation |
| drug | DRUGBANK | Medroxyprogesterone acetate |
| disease | MESH | thrombosis |
| pathway | KEGG | Platelet activation |
| drug | DRUGBANK | PAC-1 |
| disease | MESH | COVID-19 |
| disease | MESH | myocardial infarction |
| drug | DRUGBANK | Ticagrelor |
| drug | DRUGBANK | Acetylsalicylic acid |
| drug | DRUGBANK | Acetohydroxamic acid |
| disease | MESH | Atherosclerosis |
| disease | MESH | Cardiovascular disease |
| disease | MESH | Heart Disease |