Publication date: Jul 01, 2026
Swine acute diarrhea syndrome coronavirus (SADS-CoV) causes severe economic losses to the swine industry. This study aimed to identify host targets differentially expressed between virulent and attenuated SADS-CoV infections via multi-omics analysis for antiviral drug development. IPI-FX cells were infected with SADS-CoV virulent P17 or attenuated P117 strain, followed by transcriptomic and proteomic analyses to identify differentially expressed mRNAs (DEMs) and proteins (DEPs). Integrated multi-omics analysis identified candidate genes with significant differences between the P17-infected group and both the control and P117-infected groups, but no differences between the P117-infected and control groups. A total of 374 DEMs and 225 DEPs (P117 vs. control), and 927 DEMs and 404 DEPs (P17 vs. control) were detected, with 58 mRNA-protein correlation pairs enriched in pathways related to protein processing, signal transduction, and immune responses. Knockdown of RNGTT significantly inhibited P17 but not P117 replication, indicating RNGTT as a key host target for virulent SADS-CoV. This study provides new insights into SADS-CoV-host interactions and a potential target for combining attenuated vaccines with antiviral agents.
Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | diarrhea |
| disease | MESH | syndrome |
| disease | MESH | infections |
| disease | MESH | strain |
| pathway | REACTOME | Signal Transduction |
| disease | MESH | Coronavirus Infections |
| disease | MESH | Swine Diseases |