Epidemiology, Clinical Spectrum, and Serotype Distribution of NS1-Confirmed Dengue Cases in Andhra Pradesh, India: A Decade-Long Retrospective Study.

Publication date: May 01, 2026

Dengue is a major public health concern in India, showing marked seasonal variation and diverse clinical manifestations. Long-term regional data are essential for improving early diagnosis and guiding public health interventions. This study assessed decade-long epidemiological trends, clinical spectrum, and dengue virus serotype distribution among NS1-confirmed dengue cases in Andhra Pradesh, India. We conducted a retrospective observational study of 30,086 clinically suspected dengue cases tested for dengue NS1 antigen at a tertiary-care center in Andhra Pradesh, India, between January 2015 and December 2024. Demographic characteristics, urban-rural residence, temporal trends, clinical manifestations classified according to the WHO 2009 dengue classification, and dengue virus serotypes were analyzed. Associations were assessed using chi-square tests, and selected comparisons of clinical manifestation categories were performed using proportion-based analyses. Among 30,086 suspected cases, 3,899 (13. 0%) were NS1-positive. Positivity was higher among males (2,142; 13. 6%), children aged ≤18 years (2,235; 20. 7%), and rural residents (1,679; 14. 7%). Dengue transmission showed consistent post-monsoon peaks from August to October, with a marked decline in 2020 during COVID-19-related movement restrictions. Severe dengue with hemorrhagic manifestations was the most common clinical category (1,734; 44. 47%), followed by systemic manifestations (727; 18. 64%), neurological involvement (690; 17. 68%), gastrointestinal warning signs (307; 7. 87%), and hepatic involvement (160; 4. 10%). Neurological manifestations were significantly more frequent than gastrointestinal or hepatic involvement. Serotyping of 300 NS1-positive samples showed predominance of dengue virus serotype 3 (111; 37. 0%), followed by serotype 2 (70; 23. 3%), with mixed serotype infections (28; 9. 3%). This decade-long analysis highlights pronounced seasonality, a substantial pediatric burden, rural predominance, evolving dengue virus serotype circulation, and a notable contribution of neurological manifestations to dengue morbidity in southern India. Strengthened pre-monsoon vector control, early syndromic recognition particularly of neurological involvement, and continued molecular serotype surveillance are essential to reduce dengue-related morbidity in endemic regions.

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Concepts Keywords
Decade dengue
Hepatic epidemiology
Monsoon neurological manifestations
Rural ns1 antigen
seasonal trends
serotyping
who

Semantics

Type Source Name
disease MESH NS1
disease MESH Dengue
disease MESH COVID-19
disease MESH Severe dengue
disease MESH Neurological manifestations
disease MESH infections
pathway REACTOME Reproduction
drug DRUGBANK Coenzyme M
disease MESH hemorrhage
disease MESH shock
disease MESH encephalitis
disease MESH seizures
disease MESH included
disease MESH fever
disease MESH headache
disease MESH myalgia
disease MESH arthralgia
disease MESH pain
disease MESH abdominal pain
disease MESH vomiting
disease MESH diarrhea
disease MESH encephalopathy
disease MESH somnolence
disease MESH jaundice
disease MESH liver dysfunction

Original Article

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